Figure 7.

Neonatal Ex-4 effect on NPY and POMC fiber density in the PVH is reversed by Sim1Cre Glp1r deletion. Histological analysis of orexigenic neuropeptides NPY (A, D, G, J) and AgRP (B, E, H, K) and the anorexigenic neuropeptide POMC (M−P) evaluated in the hypothalamus of 4 week old Glp1r^loxP/loxP (control) and Sim1Cre;Glp1r^loxP/loxP female mice. IR of NPY, AgRP and POMC quantified using ImageJ (Q–R). Values are means ± SEM n = 4–6 mice/group. Scale Bar 150 μm. *, p < 0.05, **, p < 0.01 relative to Veh Glp1r^loxP/loxP by (Two-way ANOVA with Tukey Posthoc test).