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. 2017 May 17;6(7):664–680. doi: 10.1016/j.molmet.2017.05.007

Figure 3.

Figure 3

β-Cell mass is preserved in NOD-IGF1 transgenic mice. (A) Immunohistochemical detection of insulin (brown) in pancreas from 4-week-old NOD and NOD-IGF1 mice (n = 6/group). Original magnification: ×20 (pancreas, scale bar: 500 μm) and ×400 (islets, scale bar: 50 μm). (B–C) Quantification of β-cell mass (B) and number of islets/pancreatic area (C) in 4-week-old NOD and NOD-IGF1 mice (n = 6/group). (D–G) Immunohistochemical detection of insulin (D, F) and β-cell mass quantification (E, G) in normoglycemic NOD (NOD NG), hyperglycemic NOD (NOD HG), and NOD-IGF1 mice aged 15 and 30 weeks, respectively (n = 3–7/group). Original magnification ×400 (scale bar: 50 μm). (H) Double immunostaining for insulin (red) and Ki67 (green) of islets from 4-week-old NOD and NOD-IGF1 mice (n = 5/group). Blue, nuclei. Arrowheads indicate Ki67+ β-cells. Original magnification ×400 (scale bar: 50 μm). The histogram depicts the quantification of the % of Ki67 positive β-cells. (I) TUNEL staining (green) of islets from 8-week-old NOD and NOD-IGF1 mice (n = 6/group). Non-β cells were immunostained with anti-glucagon, anti-somatostatin, and anti-pancreatic polypeptide cocktail (red). Blue, nuclei. Arrowheads indicate TUNEL+ β cells. Original magnification ×400 (scale bar: 50 μm). The histogram depicts the quantification of the number of TUNEL positive β-cells in these islets (n = 6/group). Results are expressed as mean ± SEM. *P < 0.05 vs. NOD NG; #P < 0.05 vs. NOD HG.