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. 2017 May 31;2(6):729–734. doi: 10.1021/acssensors.7b00239

Figure 2.

Figure 2

Emission ratio of the caspase sensors C3-, C8-, C9-BRET (1 μM) after 5 min incubation in the absence of caspase (left 3 bars) and in the presence of caspase-3, -8, or -9 protein (1 μM). All measurements were conducted in buffer containing 20 mM Na2HPO4, 150 mM NaCl, 1 mM EDTA, 2 mM TCEP, 1 mg/mL bovine serum albumin, and 1 M sodium acetate to aid caspase dimerization, at pH = 7.1. Error bars represent the standard deviation (n = 3).