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. 2005 Jan 31;102(6):2162–2167. doi: 10.1073/pnas.0406976102

Fig. 3.

Fig. 3.

Ciphergen SELDI-MS analysis of Huα-Syn species. (A) Representative mass traces from the Ciphergen SELDI-TOF MS analysis of Huα-Syn from COS-1 cells, neurologically normal human brain, brain from A53T Huα-Syn Tg mice, and SH-SY5Y cells show the peaks corresponding to α-SynFL and α-Syn12. Analysis of artificially truncated Huα-Syn species expressed in COS-1 cells also are shown as follows: COS-1 composite, Sn120 (amino acids 1-120), Sn123 (amino acids 1-123), Sn130 (amino acids 1-130), and Sn FL (amino acids 1-140). The arrow indicate the apparent mass of the recombinant α-SynFL obtained with SELDI-TOF-MS (rSn, 14,471.1 Da). (B) Representative mass traces and the average masses of the α-Syn tryptic peptides analyzed by the SAX2 chip. Human brain-derived α-SynFL and α-Syn12 show distinct patterns of the acidic tryptic peptides. The peptides (and predicted average mass) corresponding to the peaks are shown. The secondary peaks result from the oxidation of methionines (+16 Da/MSO). MS peaks corresponding to trypsin-only control (T) and the double-charged ions (*) also are indicated.