Figure 2.

Effect of parkin on the differentiation of neural stem cells. A, Neural stem cells were isolated from embryonic day 15 forebrain germinal zones from parkin mutant or Non-tg mice. Neural stem cells were differentiated into astrocytes (B) and neuronal cells (C) in vitro as described in materials and methods. Western blot analysis confirmed the expression of TUBBIII and GFAP in terminally differentiated neurons and astrocytes. β-actin was internal control. Each band is representative for three experiments. The data are expressed as the mean ± SD of three experiments. *P< 0.05 indicates significant difference from non-tg derived neural stem cells.