Figure 2.

JPH3 inhibited the growth and migration of cancer cells. (A) Representative colony formation assay by monolayer culture and quantitative analysis. (B) Representative results of soft agar assays and quantitative analysis. (C) JPH3 expression in stably transfected cells confirmed by RT-PCR and Western blot. (D) Effect of JPH3 expression on tumor growth in nude mice. Result of tumor growth in nude mice inoculated subcutaneously with MKN-28/vector or MKN-28/JPH3 cells. *: p<0.05. (E) Migration assay with a 24-transwell system and quantitative analysis. The pictures were taken 24 h after seeding. Original magnification: 100x. (F) Wound-healing assay. Photos were taken every 24 h. Original magnification: 100x. All values are the mean ± s.d. of three independent experiments. *: p<0.05.