Fig. 2.
Nuclear DNA cleavage in nucellar cells adjacent to expanding endosperm. (A and B) Positive control treated with DNase before TUNEL assay. (C and D) TUNEL assay. Nuclei in nucellar cells adjacent to expanding endosperm are TUNEL-positive, endosperm cell nuclei are not. (A and C) Fluorescence. (B and D) Differential interference contrast (DIC) of identical areas. endo, endosperm; nuc, nucellus; arrowheads identify identical nuclei in fluorescence and DIC images. (Bar, 50 μm.)