Fig. 1.

Effect of central (A; n = 3-4) or peripheral (B; n = 2) administration of 100 μg of kisspeptin-10 (•), vehicle (▴), or 100 μg of kisspeptin-10 with acyline pretreatment (○) on GnRH release, as reflected by plasma LH levels (mean ± SEM), in agonadal juvenile male monkeys. Kisspeptin was injected at time 0 (arrow). The LH-releasing action of i.c.v. or i.v. kisspeptin was abolished by acyline pretreatment. Statistical analysis revealed significant effects of treatment, time, and treatment × time interaction.