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Changes in KiSS-1 and GPR54 mRNA content (mean ± SEM) determined by real-time PCR in MBH of intact female monkeys during pubertal development. KiSS-1 and GPR54 mRNA content increased in MBH with pubertal development. Numbers in parentheses indicate the number of animals per group. *, P < 0.005 (ANOVA), ♦, P < 0.05 (χ2 with threshold for bins set at 6,000 fg of KiSS-1 mRNA per μg of total RNA). Juv, juvenile; eP, early pubertal; mP, midpubertal.
