Fig. 2.

Purification SDS-PAGE gels of MtFBPase. Equal amounts of protein from both mutants and the wildtype were loaded on an SDS-PAGE gel (A). The purification process of T84A was documented (B). In order from left to right, the lanes are: A) Ladder (EZ Run Prestained REC protein ladder), B) T84A in exchange buffer, C) T84A eluted in buffer with 250 mM imidazole, D) second wash with 20 mM imidazole, E) first wash with 20 mM imidazole, F) second flow through sample of filtrate through Ni-NTA column, G) first flow through sample of filtrate through Ni-NTA column, H) supernatant after running through 0.45 μm filter, I) supernatant from the lysate after centrifugation and J) lysate after running on sonicator.