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Wildtype and mutant enzyme kinetic parameters.
| Sample | IC50 Li+ (μM) | ka (M−1s−1) |
kd (s−1) |
KD (μM) | Km (μM) |
Vmax (μmol min−1 mg−1) |
Kcat (s−1) |
Kcat/Km (s−1 mM−1) |
|---|---|---|---|---|---|---|---|---|
| Wt | 250 ± 40 | 112 | 0.0214 | 190 | 3.7 ± 0.1 | 3.5 ± 2.4 | 2.1 ± 1.5 | 570 |
| T84A | nd | 141 | 0.0208 | 147 | nd | nd | nd | nd |
| T84S | 280 ± 70 | 121 | 0.0172 | 142 | 5.7 ± 1.2 | 0.37 ± 0.26 | 0.22 ± 0.16 | 39 |
