Figure 3.

Eupafolin suppressed HCC cells growth and angiogenesis in vivo. Antitumor activity of eupafolin was investigated in HepG2 and Hep3B xenograft models, mice were randomly assigned into groups when tumor volume reached 100 mm³. The dosage of eupafolin was 60 mg/kg and was administrated three times per week by intraperitoneal injection. A, the growth curve of HepG2(left panel) and Hep3B (right panel) xenograft; B, the weight of tumors in vehicle and eupafolin-treated group, left: HepG2, right: Hep3B; C, tumor tissues were performed with immunohistochemistry analysis by staining with anti-Ki67, anti-CD31 antibody to detect the change of angiogenesis in tumor tissue. Left panel, representative photograph of tumor tissue per group (200×); right panel, the expression of indicated marker in per group was quantified, the asterisks (*, p<0.05, **, p<0.01, Student's t test) indicated significant difference.