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. 2017 May 16;13(6):701–711. doi: 10.7150/ijbs.17534

Figure 4.

Figure 4

Eupafolin inhibited VEGFR2 signaling pathway in HUVECs and HCC cells. A and C, eupafolin inhibited VEGFR2 phosphorylation in HUVECs and HepG2 cells. HUVECs(A) and HepG2 cells(C) were incubated with various concentrations of eupafolin for 24 h and the cell lysates were subjected to western blotting and probed with indicated antibodies, β-actin was used as loading control. B and D, eupafolin suppressed VEGF-induced VEGFR2 phosphorylation in HUVECs and HepG2 cells. HUVECs(B) and HepG2(D) cells were starved with 0.1% FBS overnight and then incubated with various eupafolin for 2 h, after stimulating with 20 ng/ml VEGF for 10 min, cell lysates were collected and western blotting was used to examine eupafolin's effect on the activity on VEGFR2, ERK1/2 and Akt, β-actin was used as loading control.