Figure 6.

qPCR-based expression profiling of three APC transporter genes and nine AAAP transporter genes in stages of B. tabaci B. Total RNA was extracted from individual eggs, nymphs, and adults of B. tabaci B, and was used for the expression analysis of AAT genes by qPCR (blue bars) and RNA-seq (black lines). The relative expression levels of each AAT gene in each developmental stage were normalized to the references gene EF-1α. The transcript levels of B. tabaci B AAT genes in different development stages were determined by calculating fragments per kilobase of exon model per million fragments mapped (FPKM) values, and results were represented by log₂(FPKM+1). E, Egg; N1-2, 1^st- and 2^nd-instar nymphs; N3, 3^rd-instar nymph; N4, 4^th-instar nymph; A, Adult.