Figure 4.

ROS accumulation is associated with DSN-induced apoptosis and migration inhibition. A) NOZ and SGC996 cells were treated with various concentrations of DSN for 4h, followed by incubation with the fluorescent probe DCFH-DA (10μM) for 30min, the ROS generation was detected using a microplate reader. B) GSH generation was determined in the absence or presence of 5mM NAC or 5mM GSH for 1h. C-D) ROS generation and cell viability were determined in the absence or presence of 5mM NAC or 5mM GSH for 1h. E) NOZ and SGC996 cells were treated in the absence or presence of NAC and GSH for 24h. Apoptosis was analysed by flow cytometry. F) Apoptosis-related protein expression in NOZ and SGC996 cells was analysed by western blot. GAPDH was used as a loading control.