Figure 6.

DSN induces GBC cell apoptosis by regulating ROS-mediated PI3K/AKT signalling. A) NOZ and SGC996 cells were treated with 4μM DSN in the absence or presence of NAC and GSH, PI3K/AKT signaling pathway-related protein expression was analysed by western blotting. B) NOZ and SGC996 cell ROS generation after transfection with CA-AKT and WT-AKT. C) NOZ and SGC996 cell ROS generation in the absence or presence of LY294002. All data are presented as the means ± standard deviations, and each experiment was repeated 3 times. Significant differences compared with the control are indicated by *p<0.05, **p<0.01, and ***p<0.001.