Fig. 3.
Rat AQP6 or mutants expressed in oocytes. X. laevis oocytes were injected with 15 ng of AQP6 WT, N60G, N60A, or N60S cRNA and cultured for 2.5 days. (A) Osmotic water permeability (Pf) of AQP6 or mutants. Arrhenius activation energy (Ea) of osmotic water permeabilities of N60G mutant oocytes and water-injected control oocytes (Inset). Osmotic-swelling assays were performed at 10, 20, or 30°C (mean ± SD, n = 5). (B) Confocal microscopy of oocytes injected with water, AQP6 WT, N60G, N60A, or N60S cRNA. The oocytes were stained by anti-rat AQP6 antibody. (C) Immunoblotting of membrane fraction from oocytes injected with water, rat AQP6 WT, N60G, N60A, or N60S cRNA.