Fig. 4.
The Gly to Asn mutants of AQP0, AQP1, AQP2, and AQP6 in oocytes. X. laevis oocytes were injected with 5 ng of AQP0, AQP1, AQP2, or the Gly to Asn mutants cRNA, or 15 ng of AQP6 N60G/G174N cRNA, and cultured for 2-3 days. (A) Osmotic water permeability (Pf) of AQP0 G49N, AQP1 G57N, AQP2 G49N, and AQP6 N60G/G174N. (B) Ion conductances of water injected, WT AQP6, or each mutant oocyte in NaCl solution (open columns) and NaNO3 solution (closed columns). Conductances were calculated from slopes of I-V curves between +10 mV and -90 mV. Only the AQP6 oocyte showed conductance increase in NaNO3 solution. (C) Confocal microscopy of oocytes injected with AQP0, AQP0 G49N, AQP1, AQP1 G57N, AQP2, AQP2 G49N, or AQP6 N60G/G174N cRNA. The oocytes were stained by the antibody against AQP0, AQP1, AQP2, or AQP6.