Fig. 1.

T-bet transactivates a highly conserved T-box half site in the IFN-γ promoter and induces IFN-γ expression. (A) Sequences of consensus T-box site and the conserved proximal IFN-γ promoter element or its mutant. (B) Reporter assays of T-bet or empty vector cotransfection with IFN-γ promoter element, or its mutants M1 and M2, were performed as in Fig. 6. After transfection, the cells were unstimulated or treated with PMA and ionomycin as indicated. Shown are the mean (± SEM) data from three independent experiments, each sample normalized to transfection efficiency by using β-galactosidase.