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. 2017 Jun 26;36:87. doi: 10.1186/s13046-017-0556-7

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Flow cytometric analysis of phenotypic changes induced by PMA treatment. Kasumi-2 cells were treated with 10⁻⁸ M PMA for 4 days (black curves) and the expression of various early B lymphoid phenotypic markers was compared to untreated cells (grey curves). Appropriate isotype-matched irrelevant antibodies were used as negative control (light grey curves). Treatment led to increased expression of the CD19, CD20, CD22 and FMC7 differentiation markers (b, c, d and e, respectively), whereas the expression of the CD10 (a) and of intracellular TdT (f) decreased