Fig. 8.

Effect of SERCA inhibition on PMA-induced expression of CD20 in Kasumi-2 cells. a: Cells were incubated with 10⁻⁸ M PMA in the absence (empty column) or in the presence (gray columns) of various thapsigargin concentrations in the sub- to low nanomolar concentration range for 4 days, and CD20 expression was measured by semi-quantitative Western blotting. Thapsigargin treatment led to a concentration-dependent inhibition of PMA-induced CD20 expression. Although higher concentrations of thapsigargin led to cell death, no major cytotoxic effects were observed at the concentrations used in these experiments (b)