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. 2017 Jun 19;7(2):44. doi: 10.3390/biom7020044

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Non-reducing sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) analysis of an affinity-purified poly Histidine-Small Ubiquitine-like Modifier protein (His-SUMO) fusion expressed in Escherichia coli (E. coli) showing the total cellular extract (Lane 1), the soluble cell extract (Lane 2), the flow-through fraction from the nickel column (Lane 3), the wash fraction (Lane 4), and the eluted fraction (Lane 5). M: molecular weight. (B) Size exclusion chromatography of the HisSUMO peptide in buffer with dithiothreitol (DTT) (blue line) and without DTT (black line).