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. 2017 Jun 26;18:35. doi: 10.1186/s12865-017-0218-8

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Main steps of IMGT/V-QUEST algorithm for the analysis of scFv sequences. ‘D1’: indicates the first identified and characterized V-(D)-J-REGION (V-DOMAIN 1). ‘D2’: indicates the second identified and characterized V-(D)-J-REGION (V-DOMAIN 2) which can be found in 3′ or in 5′ of ‘D1’ in the ‘V-orientated’ sequence