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. 2017 Jun 26;13:201. doi: 10.1186/s12917-017-1114-4

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Steps of establishing the CAM model. a Day 3 - piercing the eggshell in the blunt end of the egg. b, c Day 6 - creating a “window” in the eggshell. d Day 6 - placing the silicone ring on the CAM