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. 2017 Jun 26;17:447. doi: 10.1186/s12885-017-3413-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Expression of EMT associated genes in prostate cancer EMT. a qPCR: mRNA expression of known epithelial and mesenchymal specific genes in PC3 prostate cancer EMT model cell line. b qPCR: mRNA expression of epithelial and mesenchymal specific genes in PC3 prostate cancer cell lines previously unknown in prostate cancer EMT. * P < 0.05; ** P < 0.005; *** P < 0.0005. c Immunoblot: Protein expression of epithelial and mesenchymal specific genes in PC3 prostate cancer cell lines previously unknown in prostate cancer EMT (LSR, DPYSL3, S100A14, C1orf116, and β-actin were all probed on the same blot, so the β-actin loading control is appropriate for both Fig. 4c (LSR, DPYSL2, S100A14) and Fig. 5b (C1orf116). Data were separated into two figures for clarity)