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. 2017 Jun 26;17:447. doi: 10.1186/s12885-017-3413-3

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — C1orf116: a novel EMT regulator. a qPCR: mRNA expression of C1orf116 in EMT model prostate cancer cell lines PC3-Epi, PC3-EMT and PC3-TxR * P < 0.1; ** P < 0.05; *** P < 0.005. b Immunoblot: Protein expression of C1orf116 in EMT model prostate cancer cell lines PC3-Epi, PC3-EMT and PC3-TxR (LSR, DPYSL3, S100A14, C1orf116, and β-actin were all probed on the same blot, so the β-actin loading control is appropriate for both Fig. 4c (LSR, DPYSL2, S100A14) and Fig. 5b (C1orf116). Data were separated into two figures for clarity). c qPCR: mRNA expression of C1orf116 and other known epithelial (OVOL1, ESRP1 and CDH1) and mesenchymal (CDH2) gene in PC3-Epi cells transfected with C1orf116-siRNA relative to empty vector control * P < 0.1; ** P < 0.05; *** P < 0.005