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Expression of an Rbm3 fusion protein increases protein synthesis in N2a cells. (A) Immunoblot analysis of Rbm3 in control and c-Myc-Rbm3 cells. Cells were grown continuously at 37°C or shifted to 32°C for 24 h. β-actin was used as a loading control. (B) Incorporation of [35S]Met/cysteine into control and c-Myc-Rbm3 cells. Data are the mean of four experiments ± SD. Measurements were normalized with respect to total protein as determined by Bradford assay.
