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. Author manuscript; available in PMC: 2018 May 1.
Published in final edited form as: Acta Biomater. 2017 Mar 23;54:249–258. doi: 10.1016/j.actbio.2017.03.032

Figure 2. Nanoparticle-mediated Olig1 and Olig2 expression on hNSCs.

Figure 2

(A) Olig1 (red) expression in hNSCs after transfection with PBAE 536 nanoparticles containing plasmid encoding Olig1, plasmid encoding Olig2-GFP, or the mixture of these two plasmids (Olig1/2; 0.5 μg/cm2 Olig1 and 0.5 μg/cm2 Olig2-GFP, respectively) at the polymer/DNA ratio of 20 w/w and the DNA dose of 1 μg/cm2 on day 2. (B) Olig2 (red) expression in hNSCs after transfection with PBAE 536 nanoparticles containing plasmid encoding Olig1, plasmid encoding Olig2-GFP, or the mixture of these two plasmids on day 2. Non-transfected cells were used as a control. DAPI was used to stain cell nuclei in blue. Scale bar = 100 μm.