Factor	Name	Source	Definition	Threshold	Maximal Value
$f_{nls}$	nlstart-factor	BAM-file	${log}_2({log}_2\left(nlstart\right)+1)+1$		3
$f_{qsm}$	qsm-factor	BAM-file	${log}_2\left({log}_2(max(qsm-13,2))\right)$	15	2.916
$f_{s5}$	score5-factor	MaxEnt	${log}_2(max(score5,1))$	1	3.56
$f_{s3}$	score3-factor	MaxEnt	${log}_2(max(score3,1))$	1	4.01
$s_{str}$	strand-sign	MaxEnt			1
The BAM-file derived factor $f_{nls}$ is positive (>0). The BAM-file derived factor $f_{qsm}$ and the two MaxEnt score factors ($f_{s5}$, $f_{s3}$) are non-negative (≥ 0). Therefore wgis = 0 exactly when $\mathit{qsm}\le 15$ or $\mathit{score}\mathit{5}\le 1$ or $\mathit{score}\mathit{3}\le 1$ (threshold). The sign of wgis is determined by the strand-sign $sgn\left(wgis\right)=sgn\left(s_{str}\right)$. Maximal observed MaxEnt scores on annotated splice-sites are 11.8 for score5 and 16.1 for score3 leading to a theoretical maximum of 124.9 for wgis (on a 64 bit operating system).