Table 4.
Primer sequences used for quantitative real-time PCR (qRT-PCR).
| Gene | GenBank Accession No. | Forward (5′–3′) | Reverse (5′–3′) |
|---|---|---|---|
| β-actin | NM_007393 | GCAAGCAGGAGTACGATGAG | CCATGCCAATGTTGTCTCTT |
| XBP1 | NM_001271730 | GAGCAGCAAGTGGTGGATTT | AAAGGGAGGCTGGTAAGGAA |
| Star | NM_011485.4 | CTTGGCTGCTCAGTATTGAC | TGGTGGACAGTCCTTAACAC |
| Cyp19a1 | NM_007810.3 | GACACATCATGCTGGACACC | CAAGTCCTTGACGGATCGTT |
| Cyp11a1 | NM_019779.3 | CGATACTCTTCTCATGCGAG | CTTTCTTCCAGGCATCTGAAC |
| Cyclin A1 | Z26580.1 | GCCTTCACCATTCATGTGGAT | TTGCTGCGGGTAAAGAGACAG |
| Cyclin B1 | NM_172301.3 | AAGGTGCCTGTGTGTGAACC | GTCAGCCCCATCATCTGCG |
| Cyclin D2 | NM_009829.3 | ACACCGACAACTCTGTGAAGC | GCCAGGTTCCACTTCAGCTTA |
| Cyclin E | NM_007633 | GTGGCTCCGACCTTTCAGTC | CACAGTCTTGTCAATCTTGGCA |
| Caspase-3 | NM_001284409.1 | TGACTGGAAAGCCGAAACTC | GCAAGCCATCTCCTCATCAG |
| Bcl-2 | NM_009741.4 | CGAGAAGAAGAGGGAATCACAGG | AATCCGTAGGAATCCCAACC |
| Has2 | NM_008216.3 | ACCCTGCCTCATCTGTGGAGA | TGTTGGTAAGGTGCCTGTCGT |
| Ptgs2 | NM_011198.3 | CTCTATCACTGGCACCCCCTG | GAAGCGTTTGCGGTACTCATT |
| CHOP | NM_007837.3 | AGCTGGAAGCCTGGTATGAGGA | AGCTAGGGACGCAGGGTCAA |