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. 2017 May 30;18(6):1160. doi: 10.3390/ijms18061160

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Glycoconjugate microarray analysis to evaluate binding to multivalent glycans. Spot pattern of the glycoconjugate microarray (ver.4.2), where 96 synthetic glycan-conjugated polyacrylamides (PAAs) and glycoproteins were immobilized to a glass slide in triplicate (A); BSA (bovine serum albumin) and PAA were included as negative controls. Position markers (Cy3-BSA) were spotted and are depicted as grey-colored closed circle. Signal patterns obtained for each of Cy3-labeled chitin-binding lectins are shown (B). For reference, positive signals are abbreviated in the right panel of each fluorescence image.

Glycoconjugate microarray analysis to evaluate binding to multivalent glycans. Spot pattern of the glycoconjugate microarray (ver.4.2), where 96 synthetic glycan-conjugated polyacrylamides (PAAs) and glycoproteins were immobilized to a glass slide in triplicate (A); BSA (bovine serum albumin) and PAA were included as negative controls. Position markers (Cy3-BSA) were spotted and are depicted as grey-colored closed circle. Signal patterns obtained for each of Cy3-labeled chitin-binding lectins are shown (B). For reference, positive signals are abbreviated in the right panel of each fluorescence image.