Figure 8.

Lectin microarray analysis to observe the effect desialylation of a serum glycoprotein, fetuin (FET). Spot pattern of the lectin microarray; 43 lectins containing the six chitin-binding lectins were spotted on a glass slide, where different colors represent different glycan-binding specificities (A); Signal patterns obtained for FET and asialo-FET (B); Regions of chitin-binding lectins are indicated with boxes with solid lines and regions lectins showing altered signals by sialidase treatment are indicated with dotted lines. Bar graph representation of signal intensities of relevant lectins both before (closed bars) and after (open bars) sialidase treatment (C). Values above bars in the chitin-binding lectins show % reduction in the signal intensities after sialidase treatment.