Table 2.
Summary of sugar-binding features of the chitin-binding lectins investigated in the present work
| Lectin | DSA a | LEL b | PWM c | STL d | UDA e | WGA f |
|---|---|---|---|---|---|---|
| Common features: Binding to chito-oligo | ||||||
| chitotriose-PA (906) | N.D. | 4.6 μM | N.D. | N.D. | 57 μM | 4.7 μM |
| chitotetraose-PA (907) | 43 μM | 0.64 μM | 53 μM | 12 μM | 3.8 μM | 4.1 μM |
| Binding to LNH/LNnH | ||||||
| LNnH (type II+ type II; 733) | 43 μM | 6.7 μM | 130 μM | 130 μM | 29 μM | 93 μM |
| LNH (type I + type II; 734) | 19 μM | 3.5 μM | 93 μM | 220 μM | 35 μM | 110 μM |
| Unique features: | ||||||
| 3 other best PA-oligosaccharides | 4.0 μM (323) | 2.9 μM (905) | 150 μM (004) | LacdiNAc-PAA (Glycoconjugate microarray) | 3.7 μM (016) | 19 μM (053) |
| 4.1 μM (418) | 10 μM (903) | 300 μM (005) | 4.0 μM (014) | 19 μM (058) | ||
| 5.2 μM (420) | 39 μM (902) | 300 μM (007) | 5.5 μM (011) | 20 μM (051) |
a DSA shows affinity to highly branched N-glycans containing intact type II LacNAc, e.g., 323, 418 and 420; b LEL shows substantial affinity to repeated LacNAc structures, e.g., 902, 903 and 905; c PWM shows relatively weak but significant affinity to a few members of high-mannose-type N-glycans, i.e., 004, 005 and 007.; d STL shows the simplest binding profile among the investigated lectins in FAC, while showing rather selective binding to LacdiNAc-PAA in glycoconjugate microarray; e UDA shows extensive binding to high-mannose-type N-glycans with the structural unit Manα1-3(Manα1-6)Manα1-6Manβ, e.g., 011, 014 and 016; f WGA shows extensive binding to not only GlcNAc-containing glycoconjugates but also those having Neu5Ac clusters. WGA also shows selective binding to hybrid-type N-glycans having bisecting GlcNAc, e.g., 051, 053 and 058. N.D.: Not Detectable.