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(a) The HPLC shift of the Cy5-B in the absorption channel of 650 nm wavelength; (b) The HPLC shift of the Cy5-BF3 (18F) in the absorption channel of 650 nm wavelength; (c) The HPLC shift of the Cy5-BF3 (18F) in the radioactivity channel of Cy5-BF3 (18F). C18 Reversed Phase LC Column (Waters, SunFire C18 Column, 100 Å, 3.5 µm, 3 mm × 100 mm, water/acetonitrile: 90/10 to 10/90, 20 min, ultrapure water and HPLC acetonitrile (Sigma, St. Louis, MO, USA)).
