Figure 3.
Effect of hypoxia on metabolism-related proteins in SK-N-AS and 2137 ρ0 cells. Upper panel shows immunoblotting of the expression of pyruvate dehydrogenase kinase 1 (PDK1), pyruvate dehydrogenase (PDH) and lactate dehydrogenase A (LDH-A) proteins after different hypoxic times; the bar chart in lower panel shows the relative changes (to β-actin) of PDK1, PDH and LDH-A proteins. The results are shown as mean ± SD of more than three separate experiments in which similar results were obtained. * Represented p < 0.05 and # represented p < 0.005 when compared to normoxic condition were determined using the one-way ANOVA.