Figure 8.

The effect of DRP1 siRNA. Cells were transiently transfected with siRNA targeted for DRP1 (DRP1 siRNA) or non-specific siRNA (Mock) for 24 h, and then incubated in normoxia or 1% O₂ for 4 h. The expression of HIF-1α, DRP1 and β-actin proteins were revealed by Western blot analysis, and the quantification analyses are shown below. β-Actin was used as a loading control. The results are shown as mean ± SD of at least three experiments. * Represented p < 0.05 and # represented p < 0.005 when compared to normoxic Mock condition were determined using the one-way ANOVA.