Table 7.
Primers Name | Sequence (5′–3′) | Position (bp) a | Amplicon Length (bp) a | |
---|---|---|---|---|
1 | MMstart | AGTTGGGACCCGGGAAGA | 1–18 | 1363 |
MMutrR | TCCACAAAACCTGTTTCAGTG | 1343–1364 | ||
2 | MM2s | TCATCAGAAAGAAGTCCTTGAG | 85–106 | 496 |
MM5as | TAGGCCAGSCCCAKGATGCCATC | 558–580 | ||
3 | MM3s | GCTCCTCCAACCTGTGGGT | 307–325 | 560 |
MM7as | CAGAGAGGTGCCKGTGTCMACAA | 844–866 | ||
4 | MM5s | GATGGCATCMTGGGSCTGGCCTA | 558–580 | 564 |
MM9as | GAAGACATCWCCMAGGATCCAA | 1100–1121 | ||
5 | MM7s | TTGTKGACACMGGCACCTCTCTG | 844–866 | 520 |
MMutrR | TCCACAAAACCTGTTTCAGTG | 1343–1364 |
a Position and amplicon length was estimated according to the human PAG-L cDNA sequence identified using RNA-seq.