. 2017 Jun 8;18(6):1227. doi: 10.3390/ijms18061227

Table 8.

Specific primers applied for identification of the human PAG-L genomic sequence.

Primers Name		Sequence (5′–3′)	Position (bp) ^a	Amplicon Length (bp) ^a
1	MMstart	AGTTGGGACCCGGGAAGA	1–18	725
1	MM2as	ATCCAGGTAGTTCTCCAGGG	706–725	725
2	MM2s	TCATCAGAAAGAAGTCCTTGAG	571–592	1789
2	MMintronBr	ATTCTCCTGCCTCAACCTCCCAA	2337–2359	1789
3	MMintronB	CTCCGCATAGCCTGATCCCTT	1180–1200	1180
3	MMintronBr	ATTCTCCTGCCTCAACCTCCCAA	2337–2359	1180
4	MMintronB3	CCTCCTGCAGATATTGTATGTCC	1429–1451	1616
4	MM3as	ACCCACAGGTTGGAGGAGCC	3025–3044	1616
5	MMintronB2	TGTGAGGAATGAAGGAAAAGATGG	2840–2863	1533
5	MMintronDr	GGTGCTGCATGTCGGGAGAA	4353–4372	1533
6	MMintronC	GCTGTAGAATAGCCCACCAGG	3381–3401	992
6	MMintronDr	GGTGCTGCATGTCGGGAGAA	4353–4372	992
7	MMintronC	GCTGTAGAATAGCCCACCAGG	3381–3401	1663
7	MMintronEr	AAGACCCTCTCCATCGCACCCA	5022–5043	1663
8	MMintronD(N)	AGTCCTGCATGAGATGAACCA	4636–4656	1284
8	MMintronEr3	CTTAAGGACTTGAGGGTGGAGGTC	5896–5919	1284
9	MMintronE3	GCACAACTCAAATGTCATCAGCCA	5178–5201	742
9	MMintronEr3	CTTAAGGACTTGAGGGTGGAGGTC	5896–5919	742
10	MMintronE3	GCACAACTCAAATGTCATCAGCCA	5178–5201	1325
10	MMintronFr2	CTGGGGGGATTCTGGAAAGCTGA	6480–6502	1325
14	MM6sens	AGTGGCAGCGTGGTGATCTTTG	6301–6322	1311
14	MM7as	CAGAGAGGTGCCKGTGTCMACAA	7589–7611	1311
15	MMintronF2	TCAGCTTTCCAGAATCCCCCCAG	6480–6502	1132
15	MM7as	CAGAGAGGTGCCKGTGTCMACAA	7589–7611	1132
16	MMintronF	TGGATGGGTGGGGAAGAAATGT	7438–7459	1650
16	MM9as	GAAGACATCWCCMAGGATCCAA	9066–9087	1650
17	MM8s	GACATCGTCTTCACCATCAAT	7822–7842	1266
17	MM9as	GAAGACATCWCCMAGGATCCAA	9066–9087	1266
18	MMintronF	TGGATGGGTGGGGAAGAAATGT	7438–7459	405
18	MM8as	ATTGATGGTGAAGACGATGTC	7822–7842	405
19	MM992	GAGCTGCATCAGTGGCTTCC	9012–9031	318
19	MMutrR	TCCACAAAACCTGTTTCAGTG	9309–9329	318

^a Position and amplicon length was estimated according to the human PAG-L gDNA identified using capillary sequencing.