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. 2017 Jun 8;18(6):1228. doi: 10.3390/ijms18061228

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Transient dual-luciferase reporter assay showing the trans-activation of BrNYC1, BrSGR1, and BrDIN1 by BrWRKY65. (A) Diagrams of the reporter and effector constructs; (B) BrWRKY65 trans-activated BrNYC1, BrSGR1, and BrDIN1 promoters. The ratio of LUC/REN of the empty vector (62-SK) plus promoter was considered as a calibrator (set as 1). The activation is indicated by the ratio of LUC to REN. Data represents the mean ± S.E. of six independent repeats. ** indicates statistically significant differences at the level of p < 0.01 tested by Student’s t-test.