Figure 2.
Effect of Syk inhibitors on platelet adhesion to VWF. (A) Whole human blood was pretreated with DMSO (control) or OXSI-2 (1 µM) or PRT-318 (1 µM) for 5 min and perfused over immobilized VWF in a flow chamber at an arterial shear rate of 1000 s−1. After washing, stably adherent platelets were observed at 20× magnification and photographed using a confocal microscope; (B) Quantification of the percentage of area covered by using Image J software (Image Processing and Analysis in Java from National Institute of Health, https://imagej.nih.gov/ij/). Shown in the figure are representative pictures from three different experiments.