Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Jun 9;18(6):1241. doi: 10.3390/ijms18061241

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

SP induced the development of tendinopathy at protein level. Western blot for the cultured healthy tenocyte with or without SP was performed with antibodies against COL3A1, COX2, and MMP1 protein. Loading control was performed with an anti-β-actin antibody. Twenty-four hours of exposure to SP resulted in strong band densities for every protein. The results were consistent with the analysis of qRT-PCR (A); Densitometric analysis, as expected, revealed that COL3A1, COX2, and MMP1 protein band intensities normalized to β-actin were increased by 203.8%, 129.3%, and 129.3% respectively in SP-treated group compared with PBS-control. Boxplot showing the median along with the first and third quartiles (B). Error bar means data range from minimum to maximum. * p < 0.05; SP: substance P.