Table 1.
Oligonucleotides used for (RT)-PCR analysis and calculated size of the products.
| Genes | Accession No. | Primer No. | Primer Pairs | Nucleotide Positions | Tm | Size (bp) | Intron Spanning |
|---|---|---|---|---|---|---|---|
| Fcgbp | NM_001122603.1 | MB1516 | CCAAAACCTGGAGATGAGGA | 6215–6234 | 60 °C | 621 | Yes |
| MB1517 | CAGGCTACGGCAGAGATAGG | 6835–6816 | |||||
| Gkn3 | NM_026860.1 | MB2656 MB2657 |
TGGTCAGCATCCGAGACAAC CATGAGTCTGGGTCCATCGT |
270–289 612–593 |
60 °C | 343 | Yes |
| Muc2 | NM_023566.3 | MB2660 MB2661 |
GCTCTTTCTTCCTACGCCCG CATGAAGGTATGGTCAGGGC |
1913–1933 2141–2122 |
60 °C | 228 | Yes |
| Nlrp12 | NM_001033431.1 | MB2606 MB2607 |
CCCGTTACTTTGTCCCCCAT CACGCTGATTGGCTCTCAAAA |
184–203 536–516 |
60 °C | 353 | Yes |
| Tlr4 | NM_021297.3 | MB1687 | AGAAAATGCCAGGATGATGC | 269–288 | 60 °C | 417 | Yes |
| MB1688 | GTCTCCACAGCCACCAGATT | 685–666 | |||||
| T. g. RH repeat region | AF487550.1 | MB2066 MB2067 |
ACTACAGACGCGATGCCGCTC CTCTCCGCCATCACCACGAGGAA |
107–127 328–306 |
60 °C | 222 |