Total RNA abundance of (a) α ENaC; (b) β ENaC; (c) γ ENaC; and (d) ROMK in laser-captured distal tubules/connecting tubules/cortical collecting ducts analyzed by one-way ANOVA. Data are from wild-type mice on a high-salt diet (n = 4), wild-type mice on a high-salt plus EPL diet (n = 3), Nedd4-2 C2 KO mice on a high-salt diet (n = 4), and Nedd4-2 C2 KO mice on a high-salt plus EPL diet (n = 4). * p < 0.01 compared with WT HS, WT HS + EPL, and KO HS + EPL + AML; ** p < 0.01 compared with WT HS + EPL, KO HS, and KO HS + EPL; *** p < 0.01 compared with WT HS, KO HS, KO HS + EPL, and KO HS + EPL + AML; **** p < 0.01 compared with WT HS, WT HS + EPL, KO HS, and KO HS + EPL + AML; ***** p < 0.01 compared with WT HS, WT HS + EPL, KO HS + EPL, and KO HS + EPL + AML. # p < 0.01 compared with WT HS + EPL, KO HS, KO HS + EPL, and KO HS + EPL + AML; ## p < 0.01 compared with WT HS, WT HS + EPL, KO HS, and KO HS + EPL; ### p < 0.01 compared with WT HS + EPL, KO HS + EPL, and KO HS + EPL + AML.