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. 2017 Jun 14;18(6):1269. doi: 10.3390/ijms18061269

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© 2017 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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FANCD1 functional assessment. (A) Cellular viability assay. Triplicate samples of gene corrected clones were assessed for viability in response to the PARP inhibitors olaparib or KU0058948. Tetrazolium dye reduction was assessed at 490 nm over four concentrations. Controls were wild type or FANCD1 null fibroblasts of equivalent passage number; (B) RAD51 nuclear translocation. Cytoplasmic (“C”) or nuclear (“N”) fractions of untreated or MMC treated cells were analyzed by Western blot for RAD51 localization. Loading controls were α-tubulin or lamin-b1 [28] for the cytoplasmic or nuclear fractions, respectively. Blots are representative of at least two experiments on each of the indicated clones.

FANCD1 functional assessment. (A) Cellular viability assay. Triplicate samples of gene corrected clones were assessed for viability in response to the PARP inhibitors olaparib or KU0058948. Tetrazolium dye reduction was assessed at 490 nm over four concentrations. Controls were wild type or FANCD1 null fibroblasts of equivalent passage number; (B) RAD51 nuclear translocation. Cytoplasmic (“C”) or nuclear (“N”) fractions of untreated or MMC treated cells were analyzed by Western blot for RAD51 localization. Loading controls were α-tubulin or lamin-b1 [28] for the cytoplasmic or nuclear fractions, respectively. Blots are representative of at least two experiments on each of the indicated clones.