Table A1.
Frequencies of cell subsets in healthy controls (HC), other demyelinating conditions (ODC), and CIS analysed by flow cytometry according to the percentage of total PBMCs, and where relevant, percentage of total B cells, monocytes, NK cells or DCs.
| Cell Type | HC (n = 19) | ODC (n = 13) | CIS (n = 18) | p Value | |||
|---|---|---|---|---|---|---|---|
| Median | IQR | Median | IQR | Median | IQR | ||
| %PBMC | |||||||
| γδ−Τ cells | 0.43 | 0.28–0.76 | 0.61 | 0.23–1 | 0.38 | 0.29–0.58 | 0.891 |
| NKT cells | 2.21 | 1.33–4.1 | 2.32 | 1.38–2.72 | 1.92 | 1.14–2.62 | 0.519 |
| Total B cells | 8.55 | 5.71–10.56 | 7.86 | 7.06–10.89 | 7.55 | 5.41–9.33 | 0.476 |
| Total DCs | 0.86 | 0.68–1.09 | 0.74 | 0.51–0.87 | 0.92 | 0.77–1.05 | 0.165 |
| Total monocytes | 14.74 | 10.87–15.71 | 9.01 | 5.33–18.1 | 15.90 | 11.08–17.95 | 0.093 |
| Total NKs | 9.59 | 6.44–11.46 | 7.72 | 4.94–9.79 | 9.66 | 7.68–11.39 | 0.374 |
| B cells (% PBMC) | |||||||
| Antibody secreting cells | 0.04 | 0.02–0.06 | 0.06 | 0.03–0.13 | 0.04 | 0.03–0.12 | 0.479 |
| CD1c− B cells | 4.43 | 3.03–6.48 | 4.26 | 3.82–5.85 | 4.34 | 2.89–5.45 | 0.687 |
| CD1c+ B cells | 2.77 a | 2.37–3.68 | 5.11 a,b | 4.06–6.56 | 2.98 b | 2.44–4 | 0.003 |
| CD24hi CD38- B cells | 0.35 | 0.28–0.5 | 0.50 | 0.23–0.55 | 0.47 | 0.44–0.67 | 0.344 |
| CD27+ B cells | 3.00 | 2.06–3.32 | 2.37 | 1.67–3.82 | 2.11 | 2–3.12 | 0.628 |
| Double negative memory B cells | 0.24 | 0.15–0.43 | 0.33 | 0.23–0.39 | 0.28 | 0.21–0.49 | 0.41 |
| Naïve B cells | 4.49 | 2.58–6.21 | 4.94 | 3.81–5.33 | 4.14 | 2.57–6.41 | 0.745 |
| Non-switched memory B cells | 1.39 | 1.06–2.01 | 0.95 | 0.5–1.85 | 1.18 | 0.94–1.48 | 0.263 |
| Switched memory B cells | 1.08 | 0.75–1.62 | 1.34 | 0.98–1.97 | 1.13 | 0.84–1.6 | 0.516 |
| Total memory B cells | 3.17 | 2.23–3.79 | 2.69 | 1.88–4.17 | 2.62 | 2.24–3.3 | 0.746 |
| Transitional B cells | 0.11 | 0.05–0.22 | 0.26 | 0.18–0.38 | 0.22 | 0.14–0.34 | 0.086 |
| Monocytes (%PBMC) | |||||||
| Classical monocytes | 11.85 | 8.7–13.1 | 7.81 | 3.91–15.46 | 12.11 | 9.19–16.13 | 0.215 |
| Intermediate monocytes | 0.65 | 0.53–1.06 | 0.47 | 0.28–0.7 | 0.85 | 0.53–1.03 | 0.101 |
| Non-classical monocytes | 1.99 a | 1.46–2.77 | 1.18 a,b | 0.98–1.64 | 1.92 b | 1.32–2.77 | 0.003 |
| NK cells (%PBMC) | |||||||
| CD56dimCD16hi NK cells | 7.61 | 4.68–10.5 | 6.76 | 3.85–8.21 | 8.31 | 6.46–10.35 | 0.397 |
| CD56dimCD16hiCD57- NK cells | 1.91 | 1.32–2.96 | 1.68 | 1.38–2.33 | 2.03 | 1.78–3.07 | 0.436 |
| CD56dimCD16hiCD57+ NK cells | 5.46 | 3.35–7.12 | 4.63 | 2.06–5.55 | 5.51 | 4.35–6.74 | 0.568 |
| CD56brightCD16int NK cells | 0.32 | 0.22–0.6 | 0.33 | 0.22–0.59 | 0.38 | 0.27–0.49 | 0.885 |
| CD56brightCD16lo NK cells | 0.49 | 0.37–0.62 | 0.36 | 0.28–0.61 | 0.41 | 0.23–0.55 | 0.333 |
| DCs (%PBMC) | |||||||
| mDC1 | 0.44 | 0.28–0.51 | 0.32 | 0.25–0.43 | 0.36 | 0.3–0.51 | 0.428 |
| mDC2 | 0.03 | 0.01–0.08 | 0.06 | 0.02–0.07 | 0.06 | 0.04–0.12 | 0.094 |
| pDC | 0.37 | 0.33–0.5 | 0.23 | 0.17–0.39 | 0.39 | 0.33–0.5 | 0.086 |
| B cells (%B cells) | |||||||
| Antibody secreting cells | 0.49 | 0.27–1 | 0.52 | 0.39–1.17 | 0.67 | 0.36–1.55 | 0.598 |
| CD1c− B cells | 56.58 a | 51.16–66.14 | 41.63 a | 38.86–60.92 | 53.42 | 48.75–66.84 | 0.018 |
| CD1c+ B cells | 43.41 a | 33.85–48.83 | 58.36 a | 39.07–61.13 | 46.57 | 33.15–51.24 | 0.018 |
| CD24hiCD38−IgD+CD27− B cells | 5.82 | 3.08–9 | 5.40 | 3.65–6.85 | 6.38 | 4.23–11.68 | 0.415 |
| CD27− B cells | 60.67 | 56.68–69.43 | 66.37 | 60.15–75.44 | 60.67 | 56.7–71.89 | 0.478 |
| CD27+ B cells | 37.72 | 30.02–39.2 | 32.21 | 21.33–39.05 | 29.96 | 26.77–39.46 | 0.532 |
| Double negative memory B cells | 3.49 | 2.18–4.58 | 4.08 | 3.33–4.67 | 4.73 | 3.02–5.89 | 0.171 |
| Naive B cells | 57.66 | 50.19–62.89 | 58.68 | 52.92–66.86 | 54.05 | 47.85–66.53 | 0.588 |
| Non-switched memory B cells | 21.18 | 11.96–24.42 | 14.67 | 4.52–19.79 | 14.11 | 11.35–22.55 | 0.316 |
| Switched memory B cells | 16.53 | 11.47–19.89 | 17.22 | 14.54–17.62 | 16.22 | 13.02–18.88 | 0.964 |
| Total memory B cells | 39.82 | 34.33–45.48 | 36.19 | 25.87–42.64 | 35.85 | 30.1–45.16 | 0.677 |
| Transitional B cells | 1.79 a | 0.88–2.54 | 3.20 | 1.5–4.37 | 2.75 a | 2.47–4.19 | 0.016 |
| Monocytes (% Monocytes) | |||||||
| All CD16+ monocytes | 21.54 | 17.11–25.82 | 22.02 | 13.28–28.39 | 18.82 | 16.76–27.1 | 0.689 |
| Classical monocytes | 78.45 | 74.17–82.88 | 77.97 | 71.6–86.71 | 77.38 | 71.28–83.23 | 0.937 |
| Intermediate monocytes | 5.68 | 4.48–7.25 | 5.54 | 4.74–7.39 | 5.48 | 4.39–7.23 | 0.962 |
| Non-classical monocytes | 15.12 | 12.19–17.98 | 10.59 | 8.23–22.23 | 11.57 | 9.34–13.76 | 0.27 |
| NK cells (% NK cells) | |||||||
| All CD57− NK cells | 40.53 | 34.44–46.76 | 44.66 | 34.81–54.67 | 35.69 | 27.45–43.83 | 0.394 |
| All CD57+ NK cells | 59.46 | 52.95–65.44 | 55.32 | 45.32–65.18 | 64.30 | 56.13–72.53 | 0.36 |
| CD56dimCD16hi NK cells | 85.24 | 72.69–87.87 | 81.50 | 78.06–88.26 | 87.98 | 83.56–90.98 | 0.304 |
| CD56dimCD16hiCD57− NK cells | 24.74 | 15.97–28.89 | 25.18 | 19.05–29.44 | 20.59 | 17.66–27.44 | 0.801 |
| CD56dimCD16hiCD57+ NK cells | 55.69 | 48.7–62.41 | 54.77 | 43.84–64.84 | 62.19 | 53.14–73.11 | 0.367 |
| CD56brightCD16lo NK cells | 5.07 | 3.95–8.67 | 5.40 | 3.52–7.51 | 3.95 | 3.09–5.76 | 0.168 |
| CD56brightCD16int NK cells | 5.32 | 2.26–7.36 | 4.73 | 3.38–6.83 | 3.34 | 2.88–5.1 | 0.504 |
| DC (%DC) | |||||||
| All mDC | 57.10 | 49.51–62.99 | 53.52 | 43.38–68.46 | 52.21 | 46.24–58.39 | 0.756 |
| mDC1 | 50.16 | 40.29–55.09 | 47.59 | 30.78–58.68 | 43.41 | 37.59–50.22 | 0.513 |
| mDC2 | 4.12 a | 2.89–7.22 | 8.57 | 5.82–9.78 | 8.79 a | 6.56–12.16 | 0.026 |
| pDC | 42.89 | 37–50.48 | 46.47 | 31.53–56.61 | 47.78 | 41.6–53.75 | 0.756 |
Groups shown were compared using a Kruskal Wallis test; significant results are indicated by bold p values and shaded background. Letter superscripts indicate groups that were significantly different from each other in post-tests with Bonferroni’s correction. IQR = interquartile range.