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Methods of packaging recombinant proteins into MVs. (a) Overexpression of a recombinant membrane protein (yellow) may lead to enrichment on the MV surface; (b) A periplasmic signal sequence (blue) can be used to direct the protein of interest (yellow) into the periplasm, whereby the protein of interest may become encapsulated into budding MVs; (c) Fusion of a protein of interest (yellow) with a membrane protein can lead to targeted display on the surface of MVs.
