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Expression patterns of ProGPR1:GPR1-EGFP during female gametophyte development. (A) Ovule at stage 2-III. No detectable GPR1-EGFP fluorescence at this stage. (B) Ovule at stage 3-I, showing GPR1-EGFP fluorescence in megaspore mother cell. (C–G) Fluorescence was detected in nucleus and cytoplasm of FG1 stage embryo sac (C); FG3 stage embryo sac (D); FG4 stage embryo sac (E); FG6 embryo sac (F); and FG7 embryo sac (G). Figure (A) to (G) shares the same scale bar as shown in (G).
