Figure 5.

Particular subtypes of CK positive CD45 negative cells. Triple fluorescence labeling of CD45, CK, and VDR was performed on 10⁶ PBMCs from patients, with parallel analysis. Distinct subtypes of CTCs (with white arrow) were observed: patient M1 (a,b) with more than 500 CTCs identified and patients M9 (c,d) and M25 (e,f) with superimposed VDR and CK labeling. VDR staining was also seen in PMBCs (with red arrow), with superimposed VDR and CD45 labeling. Original magnification, ×40. Scale bar (white bar in the upper left image), 10 μm.