Figure A3.
Murine BM leukocytes, T cells and B cells were sorted as described in Materials and Methods. (a) Murine spleen cells were stained with anti-CD3-APC and anti-CD19-PE antibodies to isolate mature T (red gate) and B (yellow gate) cells, respectively; (b) Bone marrow cells were stained with anti-CD45-FITC antibody to isolate granulocytes, using CD45/SSC-based sorting criteria (green gate). Cells were sorted using FACS-Aria (Becton Dickinson).
