Figure 2.
Surface expression levels of ALCAM, CD49d, and NOTCH2 on Eμ-Tcl1 tumor cells can be altered by BTK- and PKC-dependent signaling. M2-10B4 stromal cells were grown for 72 h on 24-well plates and irradiated. Four ×105 tumor cells/ml were added supplemented with (A) a BCR cross-linking α-IgM Ab, (B) the BTK inhibitor Ibrutinib, or (C) the PKC activator PMA at the indicated concentrations. Surface expression of ALCAM, CD49d, and NOTCH2 was assessed after 24 h of co-culture. Dead cells were excluded with 7-AAD. For each treatment 3–5 independent experiments were conducted with in total 6–10 different Eµ-Tcl1 cell clones. Representative histograms of one clone are shown. Clone-specific differences were normalized by dividing the gMFI of each treated sample with the gMFI of the corresponding untreated sample and bar diagrams represent means and SEMs of fold gMFIs. p values shown were calculated with the Wilcoxon signed rank test against a theoretical median of 1.